| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Lancefieldella rimae | TTTTGGCATGTGCGCTGG | CCGTTGGCAAAGGGAACAC | 59.66 | 59.64 | 159 | 159 |
100.00%
|
50.00%
|
| Primer 2 | Lancefieldella rimae | AAGCACTCGGAATCGGACC | TTTGCCGTTTGCCTGCAC | 59.78 | 59.89 | 248 | 129 |
100.00%
|
50.00%
|
| Primer 3 | Lancefieldella rimae | TCTGAAAGAGGCACCGGTG | TTTGCCGTTTGCCTGCAC | 59.63 | 59.89 | 210 | 129 |
100.00%
|
50.00%
|
| Primer 4 | Lancefieldella rimae | TGTCTCTCGTTGCCGAAGC | TTTGCCGTTTGCCTGCAC | 60.37 | 59.89 | 263 | 129 |
100.00%
|
50.00%
|
| Primer 5 | Lancefieldella rimae | TGGCGATTGCGTTGTTGC | AGCACGCTGCCCAATGAT | 60.05 | 60.04 | 268 | 121 |
100.00%
|
50.00%
|
| Primer 6 | Lancefieldella rimae | ACCGAAGCGCAGTCTCAA | ACACGATCGACCACGCAA | 59.27 | 59.66 | 254 | 120 |
100.00%
|
50.00%
|
| Primer 7 | Lancefieldella rimae | TCAAAGACCGAAGCGCAGT | ACACGATCGACCACGCAA | 59.93 | 59.66 | 260 | 120 |
100.00%
|
50.00%
|
| Primer 8 | Lancefieldella rimae | AGTCAAAGACCGAAGCGCA | ACACGATCGACCACGCAA | 59.93 | 59.66 | 262 | 120 |
100.00%
|
50.00%
|
| Primer 9 | Lancefieldella rimae | GTCGGCACTCAGTCAACCA | ACACGATCGACCACGCAA | 59.93 | 59.66 | 291 | 120 |
100.00%
|
50.00%
|
| Primer 10 | Lancefieldella rimae | TGTCGGCACTCAGTCAACC | ACACGATCGACCACGCAA | 59.93 | 59.66 | 292 | 120 |
100.00%
|
50.00%
|